A new method to obtain monozoosporangial isolates of sunflower downy mildew (Plasmopara halstedii)
type: Dataset
description: A new method to obtain monozoosporangial strains was applied on 9 pathotypes of Plasmopara halstedii (100, 300, 304, 314, 700, 704, 710, 707 and 714), the parasite causing sunflower downy mildew. Single zoosporangia were isolated from the surface of agar medium and placed on leaf disks on solid Knop medium. The best fungal development was obtained with leaf disks taken from the first pair of sunflower leaves when these measured from 5 to 8cm in length. The percentage success rate in obtaining monozoosporangial strains varied from 1.4 to 7.4% according to race. The percentage of disks showing sporulation depends on spore viability, the physiological state of leaves and the receptivity of the sunflower genotype. This method enabled us to confirm the presence of a new downy mildew pathotype in France: race 707.
landing page: 0181-1584
keyword: infoeu-reposemanticsarticle
keyword: isolate
keyword: journal-articles
keyword: leaf-disk
keyword: plasmopara-halstedii-monozoosporangial-strain
keyword: race
keyword: sdvsalife-sciences-q-bioagricultural-sciences
keyword: sunflower
issued: 2026-05-05T21:39:30.929747
modified: 2026-05-05T21:39:30.929752
language: en
contact point: Organization
  fn: CCSD

test_moissonnage_selune
type: Agent

A new method to obtain monozoosporangial isolates of sunflower downy mildew (Plasmopara halstedii)
type: Distribution
format: HTML
access URL: https://hal.science/hal-00964365
issued: 2026-05-05T21:39:30.949438
modified: 2026-05-05T21:39:30.918264