test_moissonnage_selune
type: Agent

Low Molecular Weight Glutenins in Wheat-Dependant, Exercise-Induced Anaphylaxis: Allergenicity and Antigenic Relationships with Omega 5-Gliadins
type: Distribution
access URL: https://hal.science/hal-00964249
format: HTML
issued: 2026-05-05T21:43:38.896214
modified: 2026-05-05T21:43:38.848525

Low Molecular Weight Glutenins in Wheat-Dependant, Exercise-Induced Anaphylaxis: Allergenicity and Antigenic Relationships with Omega 5-Gliadins
type: Dataset
contact point: Organization
  fn: CCSD
keyword: wheat-allergy
keyword: exercise-induced-anaphylaxis
keyword: sdvsalife-sciences-q-bioagricultural-sciences
keyword: immunoblot-inhibition-method
keyword: infoeu-reposemanticsarticle
keyword: recombinant-proteins
keyword: ige-binding
keyword: 5-gliadins
keyword: wheat-dependant
keyword: low-molecular-weight-glutenins
keyword: skin-prick-test
keyword: journal-articles
landing page: 1018-2438
description: Background: Adults suffering from wheat-dependant, exercise-induced anaphylaxis (WDEIA) develop IgE directed against wheat ω5-gliadins (major allergens for this allergy) and against wheat low-molecular weight glutenin subunits (LMW-GS). However, the ability of LMW-GS to trigger an inflammatory response is still unknown. It also remains to be determined if IgE from these patients bind the same epitopes on LMW-GS and ω5-gliadins or if the epitopes are independent. Methods: WDEIA patients were selected and skin prick tests (SPTs) were performed on them using commercial gluten, wheat flour extracts, prolamin fractions and a purified natural LMW-GS P42. The IgE-binding ability of natural and recombinant wheat prolamins was verified by immunoblot experiments. Cross-reactivity between LMW-GS and ω5-gliadins was studied by immunoblot inhibition experiments, using purified natural ω5-gliadin as an inhibitor. Results: Patients developed positive SPTs with natural LMW-GS fractions and/or with the purified LMW-GS P42. Natural and recombinant LMW-GS were highly reactive with patient IgE in immunoblot experiments, as was ω5-gliadin. However, differences in reactivity were evident within the LMW-GS group. Except for one recombinant LMW-GS (P73), IgE cross-reactivity between LMW-GS and natural ω5-gliadin was only partial. Conclusion: LMW-GS are able to promote local inflammation and they share common epitopes with ω5-gliadins. The nature of these epitopes is discussed. LMW-GS also carried specific epitopes, completely independent from the ω5-gliadin epitopes. Thus, LMW-GS behaved partly as independent allergens.
issued: 2026-05-05T21:43:38.863000
language: en
modified: 2026-05-05T21:43:38.863004