Identification and quantification of phosphatidylcholines containing very long chain polyunsaturated fatty acid (VLC-PUFA) in bovine and human retina by liquid chromatography/tandem mass spectrometry
type: Dataset
description: The retina is one of the vertebrate tissues with the highest content in polyunsaturated fatty acids (PUFA). A large proportion of the retinal glycerophospholipids, especially those found in photoreceptor membranes, are dipolyunsaturated molecular species. Among them, dipolyunsaturated phosphatidylcholine molecular species are known to contain very long chain polyunsaturated fatty acids (VLC-PUFA) from the n-3 and n-6 series and having 24 to 36 carbon atoms (C24 to C36) and four to six double bonds. Recent interest in the role of VLC-PUFA arose from the findings that a protein named ELOngation of Very Long chain fatty acids 4 (ELOVL4) is involved in their biosynthesis and that mutations in ELOVL4 gene are associated with Stargardt-like macular dystrophy (STD3), a dominantly inherited junevile macular degeneration leading to vision loss. The aim of the present work was to develop an HPLC-ESI-MS/MS method for the structural characterization and the quantification of dipolyunsaturated phosphatidylcholine (PC) molecular species containing VLC-PUFA and validated this methodology on retinas from bovines and human donors. Successful separation of phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidylcholine (PC), lyso-phosphatidylcholine (LPC) and sphingomyeline (SM) was achieved by using a silica gel column and a gradient of hexane/isopropanol/water containing ammonium formate as a mobile phase. A complete structural characterization of intact phosphatidylcholine species was obtained by collision-induced dissociation (CID) in the negative mode. Fatty acid composition and distribution can be clearly assigned based on the intensity of sn-2/sn-1 fragment ions. The characterization of PC species was done on bovine retina. Among them, 28 were dipolyunsaturated PC species containing one VLC-PUFA (C24 to C36) with three to six double bonds. VLC-PUFA was always in the sn-1 position whilst PUFA at the sn-2 position was exclusively docosahexa…
issued: 2026-05-07T04:27:37.176444
language: en
modified: 2026-05-07T04:27:37.176448
contact point: Organization
  fn: CCSD
keyword: adsorption-chromatography
keyword: analysis-method
keyword: animal
keyword: artiodactyla
keyword: bovine
keyword: complex-lipid
keyword: conference-papers
keyword: coupled-method
keyword: electrospray
keyword: eye
keyword: hplc-chromatography
keyword: human
keyword: infoeu-reposemanticsconferenceobject
keyword: long-chain
keyword: mammalia
keyword: normal-phase-chromatography
keyword: phosphatidylcholine
keyword: phospholipid
keyword: polyunsaturated-fatty-acid
keyword: qualitative-analysis
keyword: quantitative-analysis
keyword: retina
keyword: sdvmheplife-sciences-q-biohuman-health-and-pathology
keyword: tandem-mass-spectrometry
keyword: ungulata
keyword: vertebrata
landing page: 27. Journées françaises de spectrométrie de masse

Identification and quantification of phosphatidylcholines containing very long chain polyunsaturated fatty acid (VLC-PUFA) in bovine and human retina by liquid chromatography/tandem mass spectrometry
type: Distribution
format: HTML
issued: 2026-05-07T04:27:37.178688
modified: 2026-05-07T04:27:37.151400
access URL: https://hal.science/hal-00939568

test_moissonnage_selune
type: Agent