Encephalomyocarditis virus 2A protein is required for viral pathogenesis and inhibition of apoptosis
type: Dataset
description: The encephalomyocarditis virus (EMCV), a Picornaviridae virus, has a wide host spectrum and can cause various diseases. EMCV virulence factors, however, are as yet ill defined. Here, we demonstrate that the EMCV 2A protein is essential for the pathogenesis of EMCV. Infection of mice with the B279/95 strain of EMCV resulted in acute fatal disease, while the clone C9, derived by serial in vitro passage of the B279/95 strain, was avirulent. C9 harbored a large deletion in the gene encoding the 2A protein. This deletion was incorporated into the cDNA of a pathogenic EMCV1.26 strain. The new virus, EMCV1.26_2A, was capable of replicating in vitro, albeit more slowly than EMCV1.26. Only mice inoculated with EMCV1.26 triggered death within a few days. Mice infected with EMCV1.26_2A did not exhibit clinical signs, and histopathological analyses showed no damage in the central nervous system, unlike EMCV1.26-infected mice. In vitro, EMCV1.26_2A presented a defect in viral particle release correlating with prolonged cell viability. Unlike EMCV1.26, which induced cytopathic cell death, EMCV1.26_2A induced apoptosis via caspase 3 activation. This strongly suggests that the 2A protein is required for inhibition of apoptosis during EMCV infection. All together, our data indicate that the EMCV 2A protein is important for the virus in counteracting host defenses, since _2A viruses were no longer pathogenic and were unable to inhibit apoptosis in vitro.
issued: 2026-05-07T04:30:45.666024
language: en
modified: 2026-05-07T04:30:45.666029
contact point: Organization
  fn: CCSD
keyword: apoptosis
keyword: encephalomyocarditis-virus
keyword: infoeu-reposemanticsarticle
keyword: journal-articles
keyword: pathogenesis
keyword: sdvmpvirlife-sciences-q-biomicrobiology-and-parasitologyvirology
landing page: 0022-538X

Encephalomyocarditis virus 2A protein is required for viral pathogenesis and inhibition of apoptosis
type: Distribution
format: HTML
issued: 2026-05-07T04:30:45.670995
modified: 2026-05-07T04:30:45.659178
access URL: https://hal.science/hal-00939516

test_moissonnage_selune
type: Agent