Quantifying kinetics of net ion fluxes from plant tissues by non-invasive microelectrode measuring MIFE technique.
type: Dataset
contact point: Organization
  fn: CCSD
keyword: sdvbvlife-sciences-q-biovegetal-biology
keyword: mesh-salinity
keyword: mesh-ion-selective-electrodes
keyword: journal-articles
keyword: mesh-salt-tolerance
keyword: mesh-plant-cells
keyword: mesh-ion-transport
keyword: mesh-ions
keyword: mesh-cell-membrane
keyword: infoeu-reposemanticsarticle
keyword: salt-stress-tolerance
keyword: measuring-net-k-and-na-fluxes
keyword: mesh-electrophysiology
keyword: mesh-biosensing-techniques
keyword: mesh-kinetics
landing page: 1064-3745
description: Non-invasive microelectrode ion flux measuring (the MIFE system) allows concurrent quantification of net fluxes of several ions with high spatial (several μm) and temporal (ca 5 s) resolution. Over the last 10 years, the MIFE system has been widely used to study various aspects of salt stress signaling and adaptation in plants. This chapter summarizes some major findings in the area such as using MIFE for deciphering the specific and non-specific components of salinity stress, resolving the role of the plasma membrane H(+)-pump in salinity responses, proving K(+) homeostasis as a key feature of salinity tolerance, and discovering the mechanisms behind the ameliorative effects of Ca(2+) and other mitigating factors (such as polyamines or compatible solutes). The full protocols for microelectrode fabrication, calibration, and use are then given, and two basic routines for measuring net K(+) and Na(+) fluxes from salinity stressed roots are described in the context of plant screening for salt stress tolerance.
issued: 2026-05-07T17:33:59.344231
language: en
modified: 2026-05-07T17:33:59.344236

Quantifying kinetics of net ion fluxes from plant tissues by non-invasive microelectrode measuring MIFE technique.
type: Distribution
access URL: https://hal.science/hal-00921510
format: HTML
issued: 2026-05-07T17:33:59.354545
modified: 2026-05-07T17:33:59.330462

test_moissonnage_selune
type: Agent