Resistance in Escherichia coli: variable contribution of efflux pumps with respect to different fluoroquinolones.
type: Dataset
description: AIMS: Resistance to fluoroquinolones is partially the result of a decrease in drug accumulation in Escherichia coli through different mechanisms. However, the variable contribution of these mechanisms with respect to different fluoroquinolones is poorly investigated. Therefore, the current study aimed to compare the contribution of resistance attributed to efflux-mediated mechanisms for different fluoroquinolones. METHODS AND RESULTS: Susceptibility of enrofloxacin, marbofloxacin and ciprofloxacin were compared after treatment with an efflux pump inhibitor in 17 ciprofloxacin-resistant E. coli isolates, and also the expression profile of the genes encoding the porins and efflux pumps involved in this resistance was evaluated. After treatment with the efflux pump inhibitor Phe-Arg-β-naphthylamide (PAβN), susceptibilities differed significantly between antimicrobial agents, the decrease for MIC being higher for enrofloxacin than for marbofloxacin or ciprofloxacin. AcrB expression level increased significantly (+26%) in ciprofloxacin-resistant E. coli isolates compared with ciprofloxacin-susceptible isolates, whereas the expression level decreased for ompF (-50%) and ompC (-30%). CONCLUSIONS: There was a higher contribution of resistance nodulation division (RND) efflux pumps to resistance to hydrophobic fluoroquinolones. SIGNIFICANCE AND IMPACT OF THE STUDY: Comparison between expression profile of efflux pumps and hydrophobicity of the antimicrobial agents could result in variable resistance for different fluoroquinolones.
issued: 2026-05-13T22:57:47.292651
language: en
modified: 2026-05-13T22:57:47.292658
contact point: Organization
  fn: CCSD
keyword: antibiotique
keyword: escherichia-coli
keyword: fluoroquinolone
keyword: infoeu-reposemanticsarticle
keyword: journal-articles
keyword: resistance
keyword: sdvmpbaclife-sciences-q-biomicrobiology-and-parasitologybacteriology
landing page: 1364-5072

Resistance in Escherichia coli: variable contribution of efflux pumps with respect to different fluoroquinolones.
type: Distribution
format: HTML
issued: 2026-05-13T22:57:47.332105
modified: 2026-05-13T22:57:47.266553
access URL: https://anses.hal.science/hal-00795561

test_moissonnage_selune
type: Agent