A pilot study for the intrinsic labeling of egg proteins with 15N and 13C.
type: Dataset
description: The aim of this study was to produce intrinsically and uniformly doubly (15)N-(13)C-labeled proteins. These proteins can be used as intrinsic tracers of dietary amino acids, both α-amino groups and carbon skeletons, during postprandial metabolic utilization. Two (Rhodes) laying hens were fed for 16 days with a standard poultry diet supplemented with 0, 0.2% or 0.4% of a mixture of 20 doubly (15)N-(13)C-labeled AAs. A third hen was given a non-enriched diet, as the control. The eggs laid were collected over 24 days, from 3 days before to 4 days after supplementation. The (15)N and (13)C enrichments in proteins from white and yolk were measured by EA-IRMS and GC-C-IRMS for enrichment in individual amino acids. After 10 days of supplementation, the (15)N enrichment reached an isotopic plateau at 1500 to 3000 ‰, depending on the supplementation level, in both white and yolk while the (13)C enrichment was 220 to 650 ‰ in white and was 100 to 250 ‰ in yolk. The (15)N enrichment was similar among the amino acids, except for the aromatic ones in which the enrichment was lower. The δ(13)C values were variable among amino acids in both white and yolk, ranging from 77 ‰ for tyrosine to 555 ‰ for proline with the 0.2 % supplementation level. In conclusion, the incorporation of 0.2 % labeled amino acids in the hen diet allowed us to achieve sufficient enrichment for metabolic studies. However, due to the non-homogeneity of the (13)C labeling, adequate (13)C enrichment of individual amino acids must be considered depending on the investigated metabolic pathway.
issued: 2026-05-20T10:17:59.811582
language: en
modified: 2026-05-20T10:17:59.811588
contact point: Organization
  fn: CCSD
keyword: aminoacides
keyword: analyse
keyword: chimanalchemical-sciencesanalytical-chemistry
keyword: infoeu-reposemanticsarticle
keyword: journal-articles
keyword: oeuf
keyword: residus
keyword: volaille
landing page: 0951-4198

A pilot study for the intrinsic labeling of egg proteins with 15N and 13C.
type: Distribution
format: HTML
issued: 2026-05-20T10:17:59.818309
modified: 2026-05-20T10:17:59.779313
access URL: https://anses.hal.science/hal-00692496

test_moissonnage_selune
type: Agent