Detection of Extracellular Phosphatase Activity of Heterotrophic Prokaryotes at the Single‐Cell Level by Flow Cytometry
type: Dataset
description: Monitoring cell activity using substrates, which turn fluorescent due to biological activity, allows observing the presence and dynamics of sub-populations, and provides a very valuable insight in ecological studies. The phosphatase substrate ELF97 phosphate (ELF-P) is a useful tool to detect and quantify phosphatase activity (PA) of microorganisms at the single-cell level. Most of the studies dealing with PA at the single-cell level focus on autotrophic cells and only few concern heterotrophic prokaryotes (referred as bacteria in the text). While flow cytometry is a promising tool to assess the single-cell analysis, only microscopy tools have been used until now to measure the ELF labeling associated with bacteria expressing PA. Therefore, we have developed a new protocol that enables the detection of ELF alcohol (ELFA), the product of ELF-P hydrolysis, making possible the specific identification of bacteria showing PA using flow cytometry. Keywords:
landing page: Current Protocols in Cytometry
keyword: aquatic-heterotrophic-bacteria
keyword: book-sections
keyword: cell-concentration
keyword: ectoenzyme
keyword: flow-cytometry
keyword: functional-measurement
keyword: infoeu-reposemanticsbookpart
keyword: infoinfo-btcomputer-science-csbiotechnology
keyword: phosphatase-activity
keyword: sdebeenvironmental-sciencesbiodiversity-and-ecology
keyword: sdvbiolife-sciences-q-biobiotechnology
keyword: sdveeecolife-sciences-q-bioecology-environmentecosystems
keyword: sdvmpbaclife-sciences-q-biomicrobiology-and-parasitologybacteriology
issued: 2026-05-20T13:21:17.096127
modified: 2026-05-20T13:21:17.096135
language: en
contact point: Organization
  fn: CCSD

test_moissonnage_selune
type: Agent

Detection of Extracellular Phosphatase Activity of Heterotrophic Prokaryotes at the Single‐Cell Level by Flow Cytometry
type: Distribution
format: HTML
access URL: https://hal.science/hal-00692148
issued: 2026-05-20T13:21:17.106295
modified: 2026-05-20T13:21:17.029822