@prefix dcat: <http://www.w3.org/ns/dcat#> .
@prefix dct: <http://purl.org/dc/terms/> .
@prefix foaf: <http://xmlns.com/foaf/0.1/> .
@prefix vcard: <http://www.w3.org/2006/vcard/ns#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .

<https://rec.harvest-normandie.data4citizen.com/dataset/oai-hal-hal-00692148v1> a dcat:Dataset ;
    dct:description """
              Monitoring cell activity using substrates, which turn fluorescent due to biological activity, allows observing the presence and dynamics of sub-populations, and provides a very valuable insight in ecological studies. The phosphatase substrate ELF97 phosphate (ELF-P) is a useful tool to detect and quantify phosphatase activity (PA) of microorganisms at the single-cell level. Most of the studies dealing with PA at the single-cell level focus on autotrophic cells and only few concern heterotrophic prokaryotes (referred as bacteria in the text). While flow cytometry is a promising tool to assess the single-cell analysis, only microscopy tools have been used until now to measure the ELF labeling associated with bacteria expressing PA. Therefore, we have developed a new protocol that enables the detection of ELF alcohol (ELFA), the product of ELF-P hydrolysis, making possible the specific identification of bacteria showing PA using flow cytometry. Keywords:
            """ ;
    dct:identifier "hal-00692148" ;
    dct:issued "2026-05-20T13:21:17.096127"^^xsd:dateTime ;
    dct:language "en" ;
    dct:modified "2026-05-20T13:21:17.096135"^^xsd:dateTime ;
    dct:publisher <https://rec.harvest-normandie.data4citizen.com/organization/cce9db95-46d9-4dc2-84b6-764215d0a002> ;
    dct:title "Detection of Extracellular Phosphatase Activity of Heterotrophic Prokaryotes at the Single‐Cell Level by Flow Cytometry" ;
    dcat:contactPoint [ a vcard:Organization ;
            vcard:fn "CCSD" ] ;
    dcat:distribution <https://rec.harvest-normandie.data4citizen.com/dataset/oai-hal-hal-00692148v1/resource/af8b2789-b387-4853-96d3-8ef4173fb100> ;
    dcat:keyword "aquatic-heterotrophic-bacteria",
        "book-sections",
        "cell-concentration",
        "ectoenzyme",
        "flow-cytometry",
        "functional-measurement",
        "infoeu-reposemanticsbookpart",
        "infoinfo-btcomputer-science-csbiotechnology",
        "phosphatase-activity",
        "sdebeenvironmental-sciencesbiodiversity-and-ecology",
        "sdvbiolife-sciences-q-biobiotechnology",
        "sdveeecolife-sciences-q-bioecology-environmentecosystems",
        "sdvmpbaclife-sciences-q-biomicrobiology-and-parasitologybacteriology" ;
    dcat:landingPage <Current%20Protocols%20in%20Cytometry> .

<Current%20Protocols%20in%20Cytometry> a foaf:Document .

<https://rec.harvest-normandie.data4citizen.com/dataset/oai-hal-hal-00692148v1/resource/af8b2789-b387-4853-96d3-8ef4173fb100> a dcat:Distribution ;
    dct:format "HTML" ;
    dct:issued "2026-05-20T13:21:17.106295"^^xsd:dateTime ;
    dct:modified "2026-05-20T13:21:17.029822"^^xsd:dateTime ;
    dct:title "Detection of Extracellular Phosphatase Activity of Heterotrophic Prokaryotes at the Single‐Cell Level by Flow Cytometry" ;
    dcat:accessURL <https://hal.science/hal-00692148> .

<https://rec.harvest-normandie.data4citizen.com/organization/cce9db95-46d9-4dc2-84b6-764215d0a002> a foaf:Agent ;
    foaf:name "test_moissonnage_selune" .