Dyed microspheres for quantification of UV dose distributions: Photochemical reactor characterization by Lagrangian actinometry
type: Dataset
description: Lagrangian actinometry represents a new method of photochemical reactor characterization. The method is based on an application of dyed microspheres, which were developed by attachment of (E)-5-[2-(methoxycarbonyl)ethenyl]cytidine (hereafter referred to as S) to polystyrene microspheres. S is a nonfluorescent molecule that when subjected to ultraviolet (UV) irradiation yields a single product, 3-beta-D-ribofuranosyl-2,7-dioxopyrido[2,3-d]pyrimidine (hereafter referred to as P), which displays a strong fluorescence signal. Dyed microspheres were subjected to UV irradiation under a collimated beam and using a single-lamp, monochromatic (low pressure Hg), continuous-flow reactor. In parallel with these experiments, a biodosimetry experiment was conducted using Bacillus subtilis spores as the challenge organism. Particle-specific fluorescence intensity measurements were conducted on samples from the collimated-beam experiments and the flow-through reactor experiments by flow cytometry. Estimates of the dose distribution delivered by the flow-through reactor for each operating condition were developed by deconvolution of data resulting from flow cytometry analysis of these samples. In conjunction with these experiments, a numerical model was developed to simulate the behavior of the reactor system. A commercially available computational fluid dynamics package was used to simulate the flow field, while line-source integration was used to simulate the irradiance field. A particle-tracking algorithm was employed to interrogate the flow and irradiance field simulations for purposes of developing particle-specific (Lagrangian) estimates of dose delivery. Dose distribution estimates from the microspheres assays and the numerical simulations were combined with the measured dose-response behavior of B. subtilis spores to yield estimates of spore inactivation in the flow-through experiments. For the range of operating conditions used in these experiments, predictions of spore ina…
issued: 2026-05-20T16:19:54.252323
language: en
modified: 2026-05-20T16:19:54.252328
contact point: Organization
  fn: CCSD
keyword: 254-nm-radiation
keyword: chemical-actinometer
keyword: dewatering
keyword: disinfection-channels
keyword: experimentation
keyword: flow
keyword: infoeu-reposemanticsarticle
keyword: iodate
keyword: iodide
keyword: journal-articles
keyword: potassium-ferrioxalate
keyword: quantum-yield
keyword: reactors
keyword: spigprocengineering-sciences-physicschemical-and-process-engineering
landing page: JOURNAL OF ENVIRONMENTAL ENGINEERING-ASCE

Dyed microspheres for quantification of UV dose distributions: Photochemical reactor characterization by Lagrangian actinometry
type: Distribution
format: HTML
issued: 2026-05-20T16:19:54.268500
modified: 2026-05-20T16:19:54.221235
access URL: https://hal.science/hal-00691764

test_moissonnage_selune
type: Agent