Hemocyte-specific responses to the peroxidizing herbicide fomesafen in the pond snail Lymnaea stagnalis (Gastropoda, Pulmonata).
type: Distribution
access URL: https://hal.science/hal-00094510
format: HTML
issued: 2026-05-06T19:33:46.814715
modified: 2026-05-06T19:33:46.760467

test_moissonnage_selune
type: Agent

Hemocyte-specific responses to the peroxidizing herbicide fomesafen in the pond snail Lymnaea stagnalis (Gastropoda, Pulmonata).
type: Dataset
contact point: Organization
  fn: CCSD
keyword: sdvtoxecolife-sciences-q-biotoxicologyecotoxicology
keyword: immunotoxicity
keyword: phagocytosis
keyword: oxidative-stress
keyword: lysosomal-fragility
keyword: journal-articles
keyword: freshwater-snail
keyword: infoeu-reposemanticsarticle
keyword: sdvimmlife-sciences-q-bioimmunology
landing page: 0269-7491
description: Responses of circulating hemocytes were studied in Lymnaea stagnalis exposed to 10, 30, 90, and 270 mg/L fomesafen for 24 and 504 h. Flow cytometry was used to quantify fomesafen-induced production of reactive oxygen species (ROS), phagocytic activity on Escherichia coli, and oxidative burst when hemocytes were challenged by E. coli or phorbol 12-myristate-13-acetate (PMA). Lysosomal membrane damage was assessed, using the neutral-red retention time (NRRT) assay. Exposure to fomesafen for 24 h resulted in increase in ROS levels and decreases in phagocytosis and the oxidative burst in PMA-stimulated hemocytes. After 504 h, intracellular levels of ROS returned to normal, but phagocytosis of E. coli was still inhibited and the associated oxidative burst significantly reduced. After both durations of exposure, decreases of NRRT indicated that lysosome membrane fragility increased with fomesafen concentration. Potential implications for the health and survival of the snails and consequences on populations are discussed.
issued: 2026-05-06T19:33:46.779849
language: en
modified: 2026-05-06T19:33:46.779854