test_moissonnage_selune
type: Agent

Selective immunoclean-up followed by liquid chromatography for the monitoring of Polycyclic Aromatic Hydrocarbon metabolites in urine at the ng.l-1 level
type: Dataset
contact point: Organization
  fn: CCSD
keyword: immunoextration
keyword: biomarker
keyword: affinity-sorbent
keyword: urine
keyword: journal-articles
keyword: pah-metabolites
keyword: chimanalchemical-sciencesanalytical-chemistry
keyword: infoeu-reposemanticsarticle
landing page: 0021-9673
description: A selective clean-up procedure using an immunosorbent (IS) was developed for the trace-level determination, in water and urine samples, of 3-benzo(a)pyrene-glucuronide (3-BP-G), a biomarker of exposure to carcinogenic polycyclic aromatic hydrocarbons (PAHs). First, three sorbents used for the immobilization of antibodies were evaluated for their ability to limit the risk of non-specific interactions and to provide a high bonding density. The best sorbent, i.e. sepharose, was used for the immobilization of two different monoclonal antibodies. The most specific antibody for 3-BP-G was applied to the selective extraction from urine providing a clean extract, an easy and reliable quantification by comparison with a classical SPE process. The sensitivity of the fluorescence associated with the selectivity of the IS provides a limit of detection up to 1.2ngl-1 in urine for 3-BP-G.
issued: 2026-05-08T09:35:09.610201
language: en
modified: 2026-05-08T09:35:09.610205

Selective immunoclean-up followed by liquid chromatography for the monitoring of Polycyclic Aromatic Hydrocarbon metabolites in urine at the ng.l-1 level
type: Distribution
access URL: https://hal.science/hal-00090022
format: HTML
issued: 2026-05-08T09:35:09.619339
modified: 2026-05-08T09:35:09.599639