Cloning, high yield over-expression, purification, and characterization of CG18594, a new PEBP/RKIP family member from Drosophila melanogaster
type: Dataset
description: The phosphatidylethanolamine-binding protein (PEBP) family is widely distributed in various species, from bacteria to mammals. These proteins seem to modulate important cell mechanisms: they control heterotrimeric G-proteins, inhibit the MAP-kinase and NFκB signaling pathways, and also serine proteases (thrombin, neuropsin, and chymotrypsin). In order to establish structure–function relationships for this family of proteins, our study focuses on PEBPs expressed within a single organism: Drosophila melanogaster, which constitutes a model system that lends itself well to establishing links between genes' expression and the corresponding proteins' functions, and to studying physiological mechanisms such as development. Here, we describe an optimized protocol for high level over-expression and high yield/high purity production of CG18594, one of Drosophila six putative PEBPs, for biophysical studies. The yield of the purified 15N labeled protein is estimated to be 60 mg/L of M9 minimal medium. Analysis of the secondary structure using circular dichroism indicates that the protein comprises mainly β-sheets at pH 7. The good dispersion of the crosspeaks on the 1H–15N HSQC spectrum provides evidence of a proper folding of the purified protein, though its time evolution suggests a tendency to denature. Taken together, these data are consistent with the assumption that the CG18594 protein belongs to the PEPB family.
landing page: 1046-5928
keyword: cg10298
keyword: cg17917
keyword: cg17919
keyword: cg18594
keyword: cg6180
keyword: cg7054
keyword: drosophila
keyword: infoeu-reposemanticsarticle
keyword: isotope-labeling
keyword: journal-articles
keyword: nmr
keyword: phosphatidylethanolamine-binding-protein
keyword: purification
keyword: rkip
issued: 2026-05-08T19:25:09.982054
modified: 2026-05-08T19:25:09.982060
language: en
contact point: Organization
  fn: CCSD

test_moissonnage_selune
type: Agent

Cloning, high yield over-expression, purification, and characterization of CG18594, a new PEBP/RKIP family member from Drosophila melanogaster
type: Distribution
format: HTML
access URL: https://hal.science/hal-00088927
issued: 2026-05-08T19:25:09.984344
modified: 2026-05-08T19:25:09.959430