@prefix dcat: <http://www.w3.org/ns/dcat#> .
@prefix dct: <http://purl.org/dc/terms/> .
@prefix foaf: <http://xmlns.com/foaf/0.1/> .
@prefix vcard: <http://www.w3.org/2006/vcard/ns#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .

<https://rec.harvest-normandie.data4citizen.com/dataset/oai-hal-hal-00087952v1> a dcat:Dataset ;
    dct:description """
              The hydrolytic activity of a monoclonal catalytic antibody (9A8) (abzyme) with acetylcholinesterase-like activity was investigated in water-in-oil (w/o) microemulsions (reverse micelles) based on sodium bis-2-(ethylhexyl)sulfosuccinate (AOT) in isooctane, using p- and o-nitrophenylacetate (p-and o-NPA) as substrates. The dependence of the abzyme hydrolytic activity on the molar ratio of water to surfactant (w(o)) showed a bell-shaped curve, presenting a maximum at w(o)=11.1. An increase of the AOT concentration at constant w(o), resulted in a decrease of the catalytic activity suggesting a possible inhibition effect of the surfactant. The incorporation of the abzyme into the reverse micelle system caused a blue shift of the fluorescence emission maximum by a magnitude of 7-10 nm depending on the w(o) value. This result indicates that the antibody molecule, or a large part of it, is located in the aqueous microphase of the system. Kinetic studies showed that the hydrolysis of p-and o-NPA in microemulsion system as well as in aqueous solution follows Michaelis-Menten kinetics. The catalytic efficiency (k(cat)/K(m)) in w/o microemulsion was significant lower than in aqueous solution.
            """ ;
    dct:identifier "hal-00087952" ;
    dct:issued "2026-05-09T04:06:51.069553"^^xsd:dateTime ;
    dct:language "en" ;
    dct:modified "2026-05-09T04:06:51.069558"^^xsd:dateTime ;
    dct:publisher <https://rec.harvest-normandie.data4citizen.com/organization/cce9db95-46d9-4dc2-84b6-764215d0a002> ;
    dct:title "Studies on the catalytic behavior of a cholinesterase-like abzyme in AOT microemulsion system" ;
    dcat:contactPoint [ a vcard:Organization ;
            vcard:fn "CCSD" ] ;
    dcat:distribution <https://rec.harvest-normandie.data4citizen.com/dataset/oai-hal-hal-00087952v1/resource/0ebc8b57-3794-4b2a-a74a-ad784f77ce75> ;
    dcat:keyword "aot-surfactant",
        "catalytic-antibody",
        "chimgenichemical-scienceschemical-engineering",
        "infoeu-reposemanticsarticle",
        "isooctane",
        "journal-articles",
        "reverse-micelle" ;
    dcat:landingPage <ISSN:%200168-1656> .

<ISSN:%200168-1656> a foaf:Document .

<https://rec.harvest-normandie.data4citizen.com/dataset/oai-hal-hal-00087952v1/resource/0ebc8b57-3794-4b2a-a74a-ad784f77ce75> a dcat:Distribution ;
    dct:format "HTML" ;
    dct:issued "2026-05-09T04:06:51.079926"^^xsd:dateTime ;
    dct:modified "2026-05-09T04:06:51.059054"^^xsd:dateTime ;
    dct:title "Studies on the catalytic behavior of a cholinesterase-like abzyme in AOT microemulsion system" ;
    dcat:accessURL <https://hal.science/hal-00087952> .

<https://rec.harvest-normandie.data4citizen.com/organization/cce9db95-46d9-4dc2-84b6-764215d0a002> a foaf:Agent ;
    foaf:name "test_moissonnage_selune" .