Modulation of HSP70 GlcNAc-directed lectin activity by glucose availability and utilization
type: Dataset
description: It is well-accepted that protein quality control (occurring either after protein synthesis or after cell damage) is mainly ensured by HSP, but the mechanism by which HSP decides whether the protein will be degraded or not is poorly understood. Within this framework, it has been hypothesized that O-GlcNAc, a cytosolic and nuclear-specific glycosylation whose functions remain unclear, could take a part in the protection of proteins against degradation by modifying both the proteins themselves and the proteasome. Because the synthesis of O-GlcNAc is tightly correlated to glucose metabolism and Hsp70 was endowed with GlcNAc-binding property, we studied the relationship between GlcNAc-binding activity of both Hsp70 and Hsc70 (the nucleocytoplasmic forms of HSP70 family) and glucose availability and utilization. We thus demonstrated that low glucose concentration, inhibition of glucose utilization with 2DG, or inhibition of glucose transport with CytB led to an increase of Hsp70 and Hsc70 lectin activities. Interestingly, the response of Hsp70 and Hsc70 lectin activities toward variations of glucose concentration appeared different: Hsp70 lost its lectin activity when glucose concentration was >5 mM (i.e., physiological glucose concentration) in contrast to Hsc70 that exhibited a maximal lectin activity for glucose concentration approximately 5 mM and at high glucose concentrations. This work also demonstrates that HSP70 does not regulate its GlcNAc-binding properties through its own O-GlcNAc glycosylation.
landing page: 0959-6658
keyword: hsp70
keyword: infoeu-reposemanticsarticle
keyword: journal-articles
keyword: o-glcnac
keyword: sdvbbmlife-sciences-q-biobiochemistry-molecular-biology
issued: 2026-05-09T14:03:30.203149
modified: 2026-05-09T14:03:30.203154
language: en
contact point: Organization
  fn: CCSD

test_moissonnage_selune
type: Agent

Modulation of HSP70 GlcNAc-directed lectin activity by glucose availability and utilization
type: Distribution
format: HTML
access URL: https://hal.science/hal-00086696
issued: 2026-05-09T14:03:30.206598
modified: 2026-05-09T14:03:30.194825