Two step formation of streptavidin-supported lipid bilayers by PEG-triggered vesicle fusion. Fluorescence and atomic force microscopy characterization
type: Dataset
description: We have used fluorescence microscopy, fluorescence photobleaching recovery (FPR), and atomic force microscopy (AFM) to investigate the formation of tethered lipid bilayers on plane aluminum oxide or glass surfaces. The bilayers were assembled with the help of a two-step methodology recently proposed for microporous templates (Proux-Delrouyre et al. J. Am. Chem. Soc. 2001, 123, 8313). The first step consists of the accumulation of intact biotinylated vesicles (PC + DOPE) on a streptavidin sublayer itself immobilized on the substrate. The second step, clearly time separated, is the deliberate triggering of bilayer formation with the help of poly(ethylene glycol) (PEG), a fusion agent oflipidic vesicles. AFM and FPR measurements confirm that the vesicles do not spontaneously fuse during the first step provided that the streptavidin sublayer is present on the substrate. On the contrary, the treatment with PEG provokes the fast formation of a continuous lipid bilayer, as attested at the hundred nanometer scale by the AFM images and at the hundred micrometer scale by the lateral diffusion of a fluorescent probe (D = 2.2 x 10[-][8] cm[2] s[-][1] for NBD-DMPE at 22 °C).
landing page: 0743-7463
keyword: amorphous-materials
keyword: chimchemical-sciences
keyword: fluorescence
keyword: infoeu-reposemanticsarticle
keyword: journal-articles
keyword: molecules
keyword: oxides
keyword: vesicles
issued: 2026-05-09T19:05:53.628661
modified: 2026-05-09T19:05:53.628666
language: en
contact point: Organization
  fn: CCSD

test_moissonnage_selune
type: Agent

Two step formation of streptavidin-supported lipid bilayers by PEG-triggered vesicle fusion. Fluorescence and atomic force microscopy characterization
type: Distribution
format: HTML
access URL: https://hal.science/hal-00086064
issued: 2026-05-09T19:05:53.632962
modified: 2026-05-09T19:05:53.620234