@prefix dcat: <http://www.w3.org/ns/dcat#> .
@prefix dct: <http://purl.org/dc/terms/> .
@prefix foaf: <http://xmlns.com/foaf/0.1/> .
@prefix vcard: <http://www.w3.org/2006/vcard/ns#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .

<https://rec.harvest-normandie.data4citizen.com/dataset/oai-hal-hal-00069536v1> a dcat:Dataset ;
    dct:description """
              The mitochondrial uncoupling protein-3 is a member of the mitochondrial carrier protein family. As a homologue of the thermogenic brown fat uncoupling protein-1, it possesses a mitochondrial uncoupling activity and thus can influence cell energy metabolism but its exact biological function remains unclear. In the present study, uncoupling protein-3 was expressed in 293 cells using the tetracycline-inducible system and its impact on cell bioenergetics and responsiveness to the apoptotic stimulus was determined. The induction of uncoupling protein-3 expression in mitochondria did not lead to uncontrolled respiratory uncoupling in intact cells. However, it caused a GDP-inhibition of state 4 respiration and a GDP-induced re-polarization of the inner mitochondrial membrane in the presence of fatty acids, in agreement with its expected physiological behavior as an uncoupling protein (UCP). Uncoupling protein-3 expression did not cause apoptosis per se but increased the responsiveness of the cells to a mitochondrial apoptotic stimulus (i.e., addition of staurosporine in the culture medium). It enhanced caspase 3 and caspase 9 activation and favored cytochrome c release. Moreover, cells in which uncoupling protein-3 expression had been induced showed a higher mitochondrial Bax/Bcl-2 ratio essentially due to enhanced translocation of Bax from cytosol to mitochondria. Finally, the induction of uncoupling protein-3 also increased the sensitivity of mitochondria to open the permeability transition pore in response to calcium. It is concluded that the presence of uncoupling protein-3 in mitochondria sensitizes cells to apoptotic stimuli involving mitochondrial pathways.
            """ ;
    dct:identifier "hal-00069536" ;
    dct:issued "2026-05-19T16:30:56.702089"^^xsd:dateTime ;
    dct:language "en" ;
    dct:modified "2026-05-19T16:30:56.702096"^^xsd:dateTime ;
    dct:publisher <https://rec.harvest-normandie.data4citizen.com/organization/cce9db95-46d9-4dc2-84b6-764215d0a002> ;
    dct:title "Uncoupling protein-3 sensitizes cells to mitochondrial-dependent stimulus of apoptosis" ;
    dcat:contactPoint [ a vcard:Organization ;
            vcard:fn "CCSD" ] ;
    dcat:distribution <https://rec.harvest-normandie.data4citizen.com/dataset/oai-hal-hal-00069536v1/resource/d5276dd6-f8ce-4f3e-9855-e369f93db693> ;
    dcat:keyword "apoptosis",
        "carrier-proteins",
        "caspases",
        "cell-respiration",
        "cytochromes-c",
        "energy-metabolism",
        "gene-expression",
        "guanosine-diphosphate",
        "infoeu-reposemanticsarticle",
        "journal-articles",
        "mitochondria",
        "protein-transport",
        "proteins-c-bcl-2",
        "proto-oncogene",
        "sdvotlife-sciences-q-bioother-q-bioot" ;
    dcat:landingPage <ISSN:%200021-9541> .

<ISSN:%200021-9541> a foaf:Document .

<https://rec.harvest-normandie.data4citizen.com/dataset/oai-hal-hal-00069536v1/resource/d5276dd6-f8ce-4f3e-9855-e369f93db693> a dcat:Distribution ;
    dct:format "HTML" ;
    dct:issued "2026-05-19T16:30:56.829820"^^xsd:dateTime ;
    dct:modified "2026-05-19T16:30:56.653986"^^xsd:dateTime ;
    dct:title "Uncoupling protein-3 sensitizes cells to mitochondrial-dependent stimulus of apoptosis" ;
    dcat:accessURL <https://hal.science/hal-00069536> .

<https://rec.harvest-normandie.data4citizen.com/organization/cce9db95-46d9-4dc2-84b6-764215d0a002> a foaf:Agent ;
    foaf:name "test_moissonnage_selune" .